The Effect of Hydroalcoholic Extract of Sativa Nigella and Honey on Liver Enzymes (AST, ALT, ALP) in Adult Male Rats Following Induction of Diabetes Mellitus

Propose:Diabetes is a metabolic disease associated with hypoglycemia, hypolipidemia, and liver dysfunction.The aim of this study was to evaluate the effects of hydroalcoholic extract of black seed and honey (dozin) on liver enzymes (AST, ALT, ALP) in streptozotocin-induced diabetic rats. Material: 70 adult male rats weighing approximately 220-200 g were divided into 7  groups: Control group: did not receive any medication. The sham  group received only solvent.  Experimental group 1 and 2: Animals in this group received 2 and 4 g / kg of hydroalcoholic extract of black seed and honey (dosin), respectively. Experimental group 3 received streptozotocin intraperitoneally as diabetic group : Animals in this group received 70 mg / kg of streptozotocin intraperitoneally, experimental group 4 and 5,  Animals in this group first received streptozotocin and then 2 and 4 g / kg of extracts. Rats  were treated for 21 days and at the end of the experimental period, blood samples were collected and serum levels of liver enzymes (AST, ALT, ALP) were measured. Findings: The results show that the levels of AST and ALP enzymes in experimental groups 5 and 4, respectively, compared with experimental group 3 (diabetic group l) and in experimental groups 1 and 2, compared with control and sham groups, showed a significant decrease at the level of p <0.05. In experimental group 3 (diabetic group), the levels of AST and ALP enzymes showed a significant increase compared to control and sham groups. Conclusion:This study showed that the hydroalcoholic extract of black seed and honey (dosine) probably improves the destructive effects of diabetes on changes in liver enzymes due to its antioxidant properties.